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Invitrogen™ Mouse VCAM-1 ELISA Kit
ELISA
Brand: Invitrogen™ EMVCAM1X10
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Description
The Mouse VCAM-1 ELISA quantitates Ms VCAM-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms VCAM-1. Principle of the method The Mouse VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
Specifications
| P29533 | |
| 1.5 pg/mL | |
| VCAM-1 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Streptavidin-HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of VCAM-1 in. Samples Using a Microplate Reader | |
| Solid-phase sandwich ELISA | |
| Colorimetric | |
| Other Proteins | |
| This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6 Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 bet | |
| Mouse | |
| Colorimetric Microplate Reader | |
| CD106,Vcam-1 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 10 x 96 Tests | |
| Development | |
| 2°C to 8°C | |
| VCAM-1 (CD106) | |
| 4 hrs 45 mins |
| 2.05 to 500 pg/mL | |
| 1.5 pg/mL to 500 pg/mL | |
| VCAM-1 | |
| HRP | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| 96 Assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 22329 | |
| Mouse | |
| <10 | |
| HRP | |
| RUO | |
| Plasma 1 μL, Serum 1 μL, Supernatant 100 μL | |
| CD106, Vcam-1 | |
| 1 hrs 20 mins |
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