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Thermo Scientific™ Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL

Code produit. p-200000105 Tous les produits Thermo Scientific Produits
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Quantité:
10 Columns
2 plates
25 columns
4 plates
5 columns
50 columns
5 colonnes
Volume d’échantillon (métrique):
1000 to 4000 μL
20 to 100 μL
300 to 800 μL
4 to 10 mL
5 to 14 μL
50 to 150 μL
500 to 2000 μL
Conditionnement:
10 pièces
2 pièces
25 pièces
4 pièces
5 pièces
50 pièces
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The new and enhanced Zeba Spin and Micro Spin Desalting columns and plates are designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They provide exceptional desalting and protein recovery performance.

Thermo Scientific Zeba spin and micro spin desalting columns and plates are designed for efficient desalting and buffer exchange of protein samples. They contain a proprietary size-exclusion chromatography resin with a robust bead structure that withstands centrifugal force and helps produce better flow characteristics and increased efficiency in removing low molecular weight protein contaminants from samples. The Zeba resin performs well even with significantly diluted protein samples and eliminates over 95% of contaminants without causing protein loss.

Separating macromolecules, such as proteins from small contaminants, is often done through routine methods including gel filtration chromatography and buffer exchange. These methods are commonly used during protein purification workflows to remove small contaminants like salts, excess labels, and cross-linking reagents from samples before analysis.

The new and enhanced Thermo Scientific Zeba Spin Desalting columns and plates are specifically designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They contain a proprietary and enhanced size-exclusion chromatography resin with a more robust bead structure, making them ideal for handling centrifugal force pressure. The strengthened bead structure results in better flow characteristics and increased efficiency in removing low MW protein contaminants from samples. The Zeba resin performs exceptionally well in purifying protein samples, even when they are significantly diluted. It eliminates over 95% of contaminants without causing protein loss.

Zeba desalting columns are the ideal solution for quick and straightforward desalting and buffer exchanging of protein samples. They are offered in variousformats, such as pre-dispensed spin columns (75 μL, 0.5 mL, 2 mL, 5 mL, 10 mL), 96-well filter plates, and chromatography cartridges. These options make it simple to handle sample volumes between 2 μL and 4 mL.

Zeba Spin Desalting 96-well Plates provide trouble-free high throughput desalting and buffer exchange for sample volumes ranging from 20 to 100 μL. They require no resin dispensing or hydration, and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in five minutes.

Features and Benefits
  • High-performing resin—exceptional protein recovery at lower protein concentrations
  • Fast and Efficient—eliminate sample dilution, fraction collection, and the need for expensive chromatography system that take time to set up and can only process one sample at a time
  • Easy to use—no cumbersome column preparation or equilibration
  • Flexible formats—available in spin columns and 96-well spin plates for a range of sample volumes (2 μL to 4 mL)
TRUSTED_SUSTAINABILITY

Spécification

Formule 20 mL Zeba Desalting Resin Bed
À utiliser avec (équipement) Standard Table-top Centrifuge
À utiliser avec (application) Small molecule removal and buffer exchange
Contenu et stockage Store at 4°C
Format Spin Column
Cible de purification Buffer Exchange, Protein
Type de colonne Size-exclusion, Proprietary Resin
MWCO 40 kDa
Gamme de produits Zeba™
Jetable Single-use
Quantité 5 colonnes
Volume (métrique) 20 mL
Volume d’échantillon (métrique) 4 to 10 mL
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