Thermo Scientific™ Kits de streptavidine d’immunoaffinité (IA) SMART Digest™

Description

Obtenez une immunocapture et une digestion des protéines rapides, simples, hautement reproductibles et sensibles dans un seul puits grâce aux kits de streptavidine Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA). Ces kits ont été conçus pour relever les défis souvent associés au processus de quantification des biomarqueurs en fournissant un flux de travail simple et facile de capture-digestion qui au lieu de durer toute la nuit, ne prend que quelques heures. Le kit purifie diverses biomolécules cibles par la formation d’une forte liaison streptavidine-biotine.

Solving the Challenges of Low-level Quantitation in Complex Matrices

The need to improve the ability to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.

As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.

Immunocapture and Protein Digestion in a Single Well

These IA kits deliver a process that is:

• Fast
• Simple
• Highly reproducible
• Sensitive

Unique Design Reduces Workflow to Hours

In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.