Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Beschreibung

Thermo Scientific™ Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

Highlights:

• Increases protein detection – most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method

• Improves antibody binding – the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively

• Works for nearly any protein – signal enhancement has been demonstrated with targets such as IL-6, p53, NFkB, BRCA1 and EGF

• Effective with any substrate – enhances both chemiluminescent (ECL) and colorimetric detection for Western blots

• Compatible with any membrane – enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)

• Fast, 15-minute protocol – optimized for a combination of simplicity, speed and signal enhancement for most proteins

• Ready-to-use – no formulating or diluting necessary, and the reagents are stable for storage at room temperature

Includes:

Enhancer Reagent 1, 250mL; Enhancer Reagent 2, 250mL