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Macherey-Nagel™ NucleoSpin™ cfDNA Midi Kit for cell-free DNA from plasma
Isolation of cell-free DNA from up to 5 mL blood plasma, utilizing the NucleoVac 96 Vacuum Manifold (sold separately, MPN 740681). The kit is designed for high recovery of circulating fragmented DNA ≥ 50 bp from human EDTA plasma.
Marca: Macherey-Nagel™ 740302.48
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Descrizione
The NucleoSpin™ DNA Plasma Midi kit is designed for the efficient isolation of circulating DNA from human blood plasma. Fragmented DNA of 50 bp and larger can be purified with high efficiency.
- Superior recovery of fragmented cell-free DNA
- Parallel purification of 24 samples—Adapter set for NucleoVac 96 Vacuum Manifold available
- Can be used with standard manual and automated vacuum manifolds
- Optimized protocol for Cell-Free DNA BCT™ (Streck Tubes)
- The kit is fully automatable on many liquid handling robots
- The protocol follows state-of-the-art bind—wash—elute procedures
- Ready to use for downstream applications like real-time PCR or NGS
Specifica
| Midi Spin Column | |
| Blood Plasma | |
| Cell-free DNA | |
| DNA isolation from blood plasma | |
| 48 | |
| Fragment size: ≥ 50 bp | |
| 90 min/24 preps |
| Silica Membrane Technology | |
| Elution volume: 200 μL (140 μL final eluate) | |
| Vacuum (e.g. NucleoVac 96 Vacuum Manifold and vacuum pump) | |
| NucleoSpin Plasma Midi Columns, Collection Tubes, buffers, Proteinase K | |
| Core Kit | |
| 1 - 5 mL | |
| Depends on sample, typically: 0.1 - 100 ng DNA/mL plasma |
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