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Invitrogen™ RiboPure™ RNA Purification Kit
Prétraitement enzymatique non requis
Marque: Invitrogen™ AM1924M
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Description
The Ambion™ RiboPure™ Kit is designed for rapid purification of high-quality RNA from tissue samples or cultured cells. The kit combines TRI Reagent™ with glass fiber filter purification to yield exceptionally pure RNA, free of residual proteins and lipids. The kit includes sufficient reagents for 50 purifications from 1–100 mg of tissue or 0.1–20 x 106 cultured cells each.
• Yields the highest purity RNA attainable
• Ideal for the most stringent downstream applications
• Handles problematic samples with ease
• Fast (less than 30 minutes) and easy procedure
RNA isolated using the RiboPure™ Kit is suitable for use in all common downstream applications, including cDNA synthesis, real-time and end-point RT-PCR, microarray analysis, northern blots, and RNase protection assays. The RiboPure™ protocol is especially suited for processing samples that are difficult to disrupt, or samples that have large amounts of ribonuclease or other contaminants. Samples are first homogenized in TRI Reagent™, a monophasic solution containing phenol and guanidine thiocyanate, to rapidly lyse cells and inactivate nucleases. Addition of bromochloropropane (BCP) results in separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, while DNA and protein remain in the interphase and organic phase. The aqueous phase is removed and passed through a glass fiber filter that binds the RNA. The filter is washed, and the RNA is eluted. The procedure is compatible with tissues that have been stored in Ambion's RNAlater™. Total RNA yield is typically 100–500 μg per 100 mg of tissue, depending on the type of tissue.
Order Info
Conditions d’expédition : Température ambiante
Spécification
| • 50 mL TRI Reagent; store at 4°C • 50 Filter Cartridges; room temperature • 100 Collection Tubes (2 mL); room temperature • 60 mL Wash Solution (concentrated); room temperature • 11 mL Elution Buffer; room temperature • Manual |
|
| 30 min. (After sample homogenization) | |
| Cells, Tissue | |
| Not High-throughput Compatible (Manual) | |
| TRI reagent, filter cartridges, 2 mL collection tubes, wash solution concentrate, elution buffer, and manual | |
| Température ambiante | |
| Jusqu’à 500 μg |
| Spin Column (Glass Fiber Filter) | |
| 100 μL | |
| ARN total | |
| RT-PCR, qPCR, construction de bibliothèques d’ADNc, NGS, analyse microarray, hybridation de transfert, transfert Northern, traduction in vitro, dosages de protection de nucléases, marquage des acides nucléiques | |
| Kit avec manuel | |
| Cells: 0.1–20 x 106 Tissue: 5–100 mg |
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Nom du produit
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
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