Invitrogen™ Kit ELISA souris pour [pS199] Tau (Phospho)

Description

The Mouse Tau (Phospho) [pS199] ELISA quantitates Ms Tau (Phospho) [pS199] in mouse cell lysates, cell culture medium and brand homogenates. The assay will exclusively recognize both natural and recombinant Ms Tau (Phospho) [pS199]. Principle of the method The Ms Tau (Phospho) [pS199] solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Favorise l’assemblage et la stabilité des microtubules et peut être impliqué dans l’élaboration et le maintien de la polarité neuronale. Le C-terminus lie les microtubules axonaux tandis que le N- terminus lie les composants de la membrane plasmique neurale, ce qui suggère que le tau fonctionne comme une protéine de liaison entre les deux. La polarité axonale est prédéterminée par la localisation TAU/MAPT (dans la cellule neuronale) dans le domaine du corps cellulaire défini par le centrosome. Les isoformes courtes permettent la plasticité du cytosquelette alors que les isoformes plus longues peuvent jouer un rôle de préférence dans sa stabilisation.

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Conditions d’expédition : Glace humide