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Gibco™ Kit de système d’expression ExpiCHO™
Description
Includes
2 flacons de cellules congelées ExpiCHO-S™, 1 flacon de 1 000 ml de milieu d’expression ExpiCHO™, 1 kit de transfection ExpiFectamine™ CHO pour 1 L de culture, 1 flacon de vecteur de témoin positif d’expression d’anticorps, 1 flacon de 100 ml d’OptiPRO™ SFM. Conservez tous les composants entre +2° et +8°C, à l’exception des cellules congelées conservées dans de l’azote liquide.
The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S cells in ExpiCHO Expression Medium. Transient expression is powered by ExpiFectamine CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed.
All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.
The ExpiCHO Expression System enables:
- Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection
- Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery
- Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
- Use of one-third to one-half less plasmid DNA than other transfection reagents
- Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
- Scalable expression for culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector.
ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately.
All components of the system are animal origin–free.
Spécification
Spécification
| Type | Système d’expression |
| Marqueur de protéine | Non marqué |
| Type de livraison | Transfection |
| Quantité | 1 kit |
| Contenu et stockage | • 2 flacons de cellules congelées ExpiCHO-S • 1 000 ml de milieu d’expression ExpiCHO • 1 kit de transfection ExpiFectamine CHO pour 1 L de culture • 1 flacon de vecteur de témoin positif d’expression d’anticorps • 100 ml d’OptiPRO SFM Conservez tous les composants entre 2 et 8°C, à l’exception des cellules congelées qui doivent être conservées dans de l’azote liquide. |
| Conditions d’expédition | approuvée pour l’expédition à température ambiante ou sur de la glace mouillée ou sèche |
| Lignée cellulaire | CHO-S, adapté au SFM |
| Système d’expression | Mammifère |
| Gamme de produits | ExpiCHO™ |
| À utiliser avec (application) | Production de protéines, expression constitutive |
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